igf1lr3-peptide The iKey Peptide BEH C18 Separation Device is a critical component in modern liquid chromatography (LC) systems, particularly for the analysis of peptidesShop iKey Peptide BEH C18 Separation Device | 300Å. This innovative device, developed by Waters, significantly simplifies microflow LC workflows by eliminating the need for traditional fittings, thereby enhancing the user experience and analytical sensitivity. Its design is central to enabling high-performance separations, especially when coupled with mass spectrometry for in-depth proteomic studies and bioanalysis.
The iKey Peptide BEH C18 Separation Device is engineered to provide robust and efficient chromatographic separations.This iKey Peptide BEH C18 Separation Devicecan resist pressures up to 10,000 psi (or 690 bar) and a pH range of 1 to 10, but we recommend that it be used ... It represents a key technology within broader systems like the ionKey/MS, which directly integrates UPLC separation into the mass spectrometer's source. This integration streamlines workflows, reduces sample handling, and improves overall analytical throughput and sensitivityAn extended stable isotope‐labeled signature peptide internal .... The device utilizes a C18 stationary phase based on Waters' Ethylene Bridged Hybrid (BEH) particle technology, renowned for its chemical stability and performance across a wide pH range.Waters iKey HT Peptide BEH C18 Separation Device, 130Ã
The iKey Peptide BEH C18 Separation Device is available in several configurations to suit diverse analytical needs. Common specifications include:
* Particle Size: Typically 1.7 µm, which contributes to high-resolution separationsTheiKey Separation deviceis available with two inner diameters: 150 µm I.D. which provides the highest level of sensitivity, and the. 300 µm I.D.iKeyHT for ....
* Pore Size: Available in 130 Å and 300 Å variationsMicrofluidic Separation Coupled to Mass Spectrometry for .... The 130 Å pore size is generally preferred for peptide separations, offering optimal access to the stationary phase for molecules within the typical peptide size range.iKey Peptide CSH C18 Separation Device, 1.7 µm, 150 ... The 300 Å option may be suitable for larger biomoleculesiKey Peptide BEH C18 Separation Device, 300Å, 1.7 µm ....
* Internal Diameter (ID): Commonly offered in 150 µm and 300 µm IDs. The 150 µm ID provides higher sensitivity due to reduced flow rates and increased analyte concentration on the column, making it ideal for low-concentration samples. The 300 µm ID, often found in "iKey HT" (High Throughput) versions, can offer faster analyses or accommodate slightly higher sample loads.Waters, 186007256, iKey BEH C18 Separation Device ...
* Length: Available in lengths such as 50 mm and 100 mm, affecting separation efficiency and analysis time.iKey Peptide BEH C18 Separation Device, 300Å, 1.7 µm, ...
* Chemical Stability: The BEH technology allows the device to withstand pressures up to 10,000 psi (approximately 690 bar) and operate within a broad pH range, typically from 1 to 10. This makes it versatile for various mobile phase conditions and sample types.
The iKey Peptide BEH C18 Separation Device is extensively used in applications demanding high sensitivity and resolution for peptide analysis. These include:
* Proteomics: Identifying and quantifying peptides from complex biological samples like cell lysates, tissues, or biofluids. This is crucial for understanding protein expression, post-translational modifications, and biological pathwaysTheiKey Separation deviceis one of the enabling technologies behind the ionKey/MS system. The innovative iKey eliminates the need for traditional fittings ....
* Bioanalysis: Developing sensitive assays for therapeutic peptides, biomarkers, or drug metabolites in biological matrices such as plasma or serum. The integration with mass spectrometry (LC-MS/MS) is particularly powerful for targeted quantification.
* Pharmaceutical Development: Characterizing peptide-based drugs, monitoring their stability, and assessing their purityImproving a High Sensitivity Assay for the Quantification of ....
* Quality Control: Ensuring the consistent quality and composition of peptide products.Shop iKey Peptide BEH C18 Separation Device | 186006969
The iKey device offers several significant advantages over traditional chromatographic setups:
* Simplified Workflow: Eliminates the need for external fittings, reducing potential leaks, dead volume, and setup complexity作者：RE Foreman·2023·被引用次数：8—The ionKey HSS T3Separation Device(100 Å, 1.8 μm, 150 μm × 50 mm, Waters) was set at 45 °C, and the analytes were separated over a 13 min .... This leads to faster method development and routine analysis.
* Enhanced Sensitivity: The microflow format inherent to the iKey system, especially with smaller internal diameters, concentrates analytes, leading to higher signal intensity when coupled with sensitive detectors like mass spectrometers.
* Robustness: The BEH particle technology provides excellent chemical and physical stability, enabling reproducible results over many injections and across a wide range of mobile phase conditions.iKey Peptide BEH C18 PCA Separation Device, 130Å, 1.7 ...
* Integration: Designed to seamlessly integrate with systems like the Waters ionKey/MS, creating a compact and efficient analytical platformUPLC separation of teriparatide and IS, from extracted plasma, using theiKey Peptide BEH C18 Separation Device, 130Å, 1.7 µm,. 150 µm x 50 mm (p/n 186006764)..
When selecting and using an iKey Peptide BEH C18 Separation Device, several factors are important:
* Pore Size: For most peptide separations, the 130 Å pore size is optimal.The ionKey/MS™ System is a robust, novel microfluidics platform that eliminates manual chromatographic connections and enables high throughput and robust ... The 300 Å version might be considered for larger peptides or small proteins, but it may offer less surface area for interaction with smaller peptides.
* Internal Diameter: Choose the 150 µm ID for maximum sensitivity, especially for trace-level analysisShop iKey Protein BEH C4 Separation Device | 186006765. The 300 µm ID (iKey HT) can be beneficial for higher throughput or slightly larger sample volumes, though it may yield lower sensitivity compared to the 150 µm versionHigh-Throughput LC-MS MRM Disease Protein Marker ....
* Column Length: Longer columns generally provide better resolution but increase backpressure and analysis time. Shorter columns are faster but may offer less resolving power.
* Mobile Phase Compatibility: While the BEH C18 phase is stable from pH 1 to 10, it's always advisable to consult specific application notes or manufacturer guidelines for optimal mobile phase compositions and gradient profiles to achieve the best separation for your target peptidesTheiKey Separation deviceis available with two inner diameters: 150 µm I.D. which provides the highest level of sensitivity, and the. 300 µm I.D.iKeyHT for ....
* System Compatibility: Ensure the iKey device is compatible with your LC and MS system, particularly the ionKey/MS interface if you are utilizing that platform.
In summary, the iKey Peptide BEH C18 Separation Device is a sophisticated and user-friendly tool that has revolutionized peptide analysis by simplifying workflows and enhancing sensitivity.TheiKey Separation deviceis available with two inner diameters: 150 µm I.D. which provides the highest level of sensitivity, and the. 300 µm I.D.iKeyHT for ... Its advanced BEH particle technology and integrated design make it a cornerstone for researchers in proteomics, bioanalysis, and pharmaceutical sciences seeking high-performance chromatographic separations.作者：RL Sayers·2017·被引用次数：37—The column andiKeywere heated to 35 °C while samples were maintained at 8 °C prior to analysis by triplicate injection of either 5 μL (conventional ...
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