PeptidePulse podcast Peptide-pulsed dendritic cells (DCs) represent a sophisticated approach in immunotherapy, leveraging the potent antigen-presenting capabilities of dendritic cells to stimulate a targeted immune response.作者：N Seishima·2023·被引用次数：1—This study aims to compare efficacy of syngeneic DC vaccines and semi-allogeneic DC vaccines and determine whether a therapeutic semi-allogeneic DC vaccine is more efficacious. This method involves loading DCs with specific peptides, which are then presented to T cells, primarily CD8+ cytotoxic T lymphocytes (CTLs), to elicit an antigen-specific immune reaction. The ability of peptide-pulsed dendritic cells to induce immune-mediated tissue destruction and enhance the quality of anti-tumor immune responses has positioned them as a promising strategy for treating various diseases, particularly cancer and chronic infections.作者：I Bedrosian·2003·被引用次数：259—Intranodal Administration of Peptide-Pulsed Mature Dendritic Cell VaccinesResults in Superior CD8+T-Cell Function in Melanoma Patients.
Dendritic cells are professional antigen-presenting cells (APCs) that play a crucial role in initiating adaptive immune responses. When pulsed with specific peptides, these DCs migrate to lymph nodes, where they present the peptide-antigen complex to naive T cells. This presentation activates T cells, leading to their proliferation and differentiation into effector cells capable of recognizing and eliminating target cells expressing the specific antigenDefinition of mutant p53 peptide pulsed dendritic cell vaccine.
The process typically involves:
* Ex vivo generation of dendritic cells: Dendritic cells are often generated from precursor cells, such as monocytes, in a laboratory setting作者：WJ LESTERHUIS·2010·被引用次数：128—To investigate the immune response against tumorpeptidesgenerated in vaccinated patients, DTH challenges were performed with mature DCs loaded with CEApeptideor CEA mRNA. Biopsies were taken from DTH induration sites, which were cultured with low amounts of IL-2 without the addition of antigen..
* Peptide pulsing: These generated DCs are then exposed to specific synthetic peptides that mimic antigens found on target cells, such as tumor cells or pathogensImmunogenicity of Dendritic Cells Pulsed with CEA Peptide ....
* Activation and maturation: The DCs are matured to enhance their ability to present antigens and migrate to lymphoid tissues.4天前—The antigen-specific T cell lines were generated by stimulating donor-derived CD8+T cells with thepeptide-pulsed DCs. The T cell lines were ...
* Administration: The peptide-pulsed, activated DCs are administered to the patient, where they prime T cells.Immunization with Epstein-Barr Virus (EBV) Peptide-pulsed ...
This strategy allows for a highly specific immune response, as the T cells are directed against the particular peptide presented by the DCs. This is particularly advantageous in cancer immunotherapy, where specific tumor-associated antigens can be identified and used to generate personalized neoantigen peptide-pulsed dendritic cell vaccines.作者：K Oyama·2025·被引用次数：7—The present study suggests the feasibility and efficacy ofNeo-P DC vaccine therapyafter surgical treatment of pancreatic cancer in both postoperative ...
The primary application of peptide-pulsed dendritic cells lies in cancer immunotherapy. By pulsing DCs with tumor-specific peptides or neoantigens, researchers aim to induce a robust anti-tumor immune response作者：K Oyama·2025·被引用次数：7—The present study suggests the feasibility and efficacy ofNeo-P DC vaccine therapyafter surgical treatment of pancreatic cancer in both postoperative .... Studies have shown that vaccines using ex vivo generated peptide-pulsed DCs can be effective in generating antigen-specific CTLs, leading to disease stabilization and potentially tumor regressionHLA I immunopeptidome of synthetic long peptide pulsed .... For instance, vaccination with WT1 peptide-pulsed dendritic cells has been investigated for its efficacy in patients with various cancers.Here we show that major histocompatibility complex class I-presentedpeptide antigen pulsed onto dendritic APCsinduces protective immunity to lethal challenge ... Similarly, personalized neoantigen pulsed dendritic cell vaccines are being developed to target unique mutations present in individual tumors.The safety and anti-tumor effect of multiple peptides-pulsed ... The combination of multiple peptide-pulsed DCs with CTLs therapy has also shown manageable safety and promising efficacy for cancer patients.Definition of mutant p53 peptide pulsed dendritic cell vaccine
Beyond cancer, peptide-pulsed dendritic cells hold potential in combating chronic infections. For example, DCs can be pulsed with peptides from pathogens like Mycobacterium tuberculosis to promote immunity protective against the infection.Vaccination with p53 peptide-pulsed dendritic cells is ... Synthetic long peptides (SLPs) are also being explored as a vaccine modality that exploits dendritic cells to treat chronic infections.
The use of peptide-pulsed dendritic cells offers several advantages:
* High specificity: The immune response is directed against precisely defined peptide antigens.Study Details | NCT00243529 | Peptide-pulsed vs. RNA- ...
* Potency: Dendritic cells are potent APCs, capable of inducing strong T cell responses.作者：S Mehrotra·2017·被引用次数：162—Dendritic cells (DCs)enhance the quality of anti-tumor immune responsein patients with cancer. Thus, we posit that DC-based immunotherapy, in ...
* Personalization: In cancer therapy, personalized vaccines can be created using neoantigens unique to a patient's tumor.
* Induction of memory: This approach can lead to the generation of memory CD8+ T cells, providing long-term immunity.
However, several factors need careful consideration:
* Route of administration: The route of immunization with peptide-pulsed, activated DCs can influence the distribution and function of memory CD8+ T cells.
* Quality and quantity of DCs: The effectiveness of the vaccine depends on the quality and number of peptide-pulsed DCs administered.
* Peptide selection: Identifying the most immunogenic and relevant peptides is crucial for vaccine efficacy.
* Tumor microenvironment: The tumor microenvironment can often suppress immune responses, posing a challenge for DC-based therapies.
Research continues to explore various aspects of peptide-pulsed dendritic cell therapy, including optimizing peptide loading, DC maturation protocols, and delivery methods. Studies comparing different vaccination strategies, such as peptide-pulsed DCs versus RNA-transfected DCs, are also underway to determine the most effective approaches for different diseasesImmunization with Epstein-Barr Virus (EBV) Peptide-pulsed .... Ultimately, peptide-pulsed dendritic cells represent a sophisticated and promising frontier in the development of immunotherapies aimed at harnessing the body's own immune system to fight disease.
Join the newsletter to receive news, updates, new products and freebies in your inbox.